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Bited a 325-bp PCR product, whereas SR-AI/II-/- mutant allele showed a 434-bp PCR product. MARCO wild type allele exhibited ca. 500-bp PCR product, and MARCO-/- mutant exhibited ca. 850-bp PCR product. All of the three cell lines are stable and Mycoplasma-free by Mycoplasma PCR ELISA test (Roche, Indianapolis, IN) during culture in the past 24 months.SR-AI/IIM WT ZK1 ZK2 ZK6 MMARCOWT ZK1 ZK2 ZKMAR
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Bited a 325-bp PCR product, whereas SR-AI/II-/- mutant allele showed a 434-bp PCR product. MARCO wild type allele exhibited ca. 500-bp PCR product, and MARCO-/- mutant exhibited ca. 850-bp PCR product. All of the three cell lines are stable and Mycoplasma-free by Mycoplasma PCR ELISA test (Roche, Indianapolis, IN) during culture in the past 24 months.SR-AI/IIM WT ZK1 ZK2 ZK6 MMARCOWT ZK1 ZK2 ZKMAR
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Surface expression of the macrophage-associated differentiation Ags was assessed by direct immunofluorescence (thick lines). ZK1 cells were incubated with FITC-labeled anti-mouse F4/80 or FITC-labeled anti-mouse CD11b. Mouse IgG2a and IgG2b were used as isotype controls (light lines). Staining of cells with FITC-labeled anti-mouse Ig compared to unstained cells detects the of cells expressing su
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Itada M, Kitagawa H, Igarashi K, Hirose S, Kanakubo Y: Polyamine lowered the hepatic lipid peroxide level in rats. Res Commun Chem Pathol Pharmacol 1988, 62:235-249. Merentie M, Uimari A, Pietil?M, Sinervirta R, Kein en TA, Veps nen J, Khomutov A, Grigorenko N, Herzig KH, J ne J, Alhonen L: Oxidative stress and inflammation in the pathogenesis of activated polyamine catabolism-induced acute pancr
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Onfim I, Camacho RJ, Vandamme AM, Lemey P: Phylodynamics of the HIV-1 CRF02_AG clade in Cameroon. Infect Genet Evol 2012, 12:453?60. 20. Zhang M, Foley B, Schultz AK, Macke JP, Bulla I, Stanke M, Morgenstern B, Korber B, Leitner T: The role of recombination in the emergence of a complex and dynamic HIV epidemic. Retrovirology 2010, 7:25. 21. Carr JK, Salminen MO, Albert J, Sanders-Buell E, Gotte D
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Hat three clones ZK1, ZK2 and ZK6 were obtained by limiting dilution and further characterized. Our PCR genotyping results verified these three clones, ZK1, ZK2 and ZK6 are MARCO-/- and SR-AI/ II-/--deficient (Fig. 1). These cell lines are able to grow rapidly in RPMI or DMEM complete media in the absence of exogenous M-CSF or other growth factors, and their doubling time is 12?6 h on the average
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One of the newly sequenced gag genes (BS72) was apparently derived through recombination between F2 and CRF36_cpx parental viruses, one of the nef genes was apparently derived through recombination between F and CRF22_01A1 parental viruses. The phylogenetic analysis of gag sequences derived from the Cameroonian samples further revealed four sequences (BS09, BS25, BS16 and BS42) situated on diverge
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Osinic acid (PolyI) and dextran sulfate (DS,Page 3 of(page number not for citation purposes)Particle and Fibre Toxicology 2008, 5:http://www.particleandfibretoxicology.com/content/5/1/WTMS-/-ZKZKModified Wright staining of ZK1 and ZK2 cell lines compared with primary alveolar macrophages Figure 3 Modified Wright staining of ZK1 and ZK2 cell lines compared with primary alveolar macrophages. Primary