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Lum stress response pathway in Oikopleura but not human cells (Additional file 1, Figure S5B). It is not clear why Endoplasmic reticulum stress response pathway is responding more in Oikopleura. This pathway can be adaptive, promoting survival or it can lead to induction of apoptosis [39]. However, Apoptosis genes respond equally in both Oikopleura and the human macrophage cells (Additional file 1
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Ologs. The second objective of the study was to examine the genome wide transcriptional responses to xenobiotic compounds using whole genome tiling arrays recently made available in Oikopleura. Oikopleura was exposed to two model xenobiotic compounds benzo[a]pyrene (BaP) and clofibrate (Clo) that are common environmental pollutants. BaP is a carcinogenic polycyclic aromatic hydrocarbon (PAH) that
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Er This property is particularly . suitable for vaccine design. There isEr This property is particularly . suitable for vaccine design. There is no limitation to the size of peptide displayed, given the ability of the E2 CD to naturally present 60 lipoyl domains plus 60 copies of the E1 (150 kDa) or E3 (100 kDa) enzymes. [61] Domingo et al demonstrated that a green fluorescentWJV|www.wjgnet.
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Analyses were done on mouse homologs of differentially regulated Oikopleura genes, but the underlying changes in gene expression or cellular processes can be relevant also in Oikopleura. For example, inspection of the modulated genes that led to the enrichment of Cystic fibrosis disease reveals that they are involved in ER stress response (e.g. HSPs). ER stress response is thought to contribute to
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Ction between ClfB and loricrin and to measure the affinity of binding, surface plasmon resonance (SPR) was employed. HLor was captured on the surface of a sensor chip that had been coated with anti-GST IgG. Previous studies have indicated that the minimum Fg and K10 binding region of ClfB comprises the N2 and N3 subdomains [27?8] (Figure S1B). Recombinant ClfB N2N3 (rClfB201?42) was expressed wit
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Re 5). Among the significantly enriched pathways, ECM-receptor interaction and Focal adhesion are also affected by BaP (Table 2). Clo had orchestrated effects on main energy pathways such as Glycolysis/Gluconeogenesis, Oxidative Phosphorylation and Citrate cycle (TCA cycle) (Table 4). While most pathways affected by Clo are presumably conserved between Oikopleura and mouse, some such as the diseas
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Ra CYPs and many CYP1 protein sequences from various organisms [28] were also used to construct a phylogenetic tree of similar topology to the one shown in Figure 1, but none of the Oikopleura CYPs cluster with CYP1 sequences (Additional file 1, Figure S1). Thus, CYP1 genes are either lost or have diverged beyond recognition in Oikopleura. In contrast, CYP1like genes are present in the genomes of
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Lycolysis/Gluconeogenesis Hypertrophic cardiomyopathy (HCM) Focal adhesion Dilated cardiomyopathy Alzheimer's disease Huntington's disease Oxidative phosphorylation Parkinson's disease Propanoate metabolism Citrate cycle (TCA cycle) Count PValue Benjamini FDR 12 9 11 9 10 11 17 11 16 16 13 13 6 6 8.28E-05 1.15E-04 2.31E-04 3.19E-04 3.74E-04 4.27E-04 8.40E-04 8.86E-04 9.98E-04 1.06E-03 1.21E-03 1.4